Molecular Detection of Newcastle Disease Virus from Field Outbreaks in Assam, India

Biological and Molecular Characterization of Newcastle Disease Virus Isolated from Iran

Molecular characterization and phylogenetic study of newcastle disease virus isolates from recent outbreaks in eastern Uganda.

Newcastle disease virus isolates from chickens in eastern Uganda in 2001 were found to be velogenic by fusion protein cleavage site sequence analysis and biological characterization; the intracerebral pathogenicity index was 1.8. Analysis of their hemagglutinin-neuraminidase protein gene sequences revealed a novel genotype unrelated to those that caused previous outbreaks.

Molecular characterization of velogenic viscerotropic Ranikhet (Newcastle) disease virus from different outbreaks in desi chickens

AIM Diagnosis of velogenic viscerotropic Ranikhet disease from six different flocks of desi chicken in and around Mumbai by gross and histopathological examination, isolation of virus and molecular methods. MATERIALS AND METHODS A total of 25 carcasses (varying between 2 and 6 carcasses from each flock) of six different flocks of adult desi chicken were subjected to necropsy examination for d...

Molecular Typing of Field Isolates from two outbreaks of Infectious Bursal Disease Virus from Pakistan

A reverse transcriptase polymerase chain reaction restriction fragment length polymorphism (RT-PCR/RFLP) technique was used for the identification and characterization of Pakistani field isolates of infectious bursal disease virus (IBDV). A total of 8 bursa samples were collected from two outbreaks during September and October 2003 from Tehsil Sumandri, Dist. Faisalabad with 40-50% mortality in...

Detection and isolation of exotic Newcastle disease virus from field-collected flies.

Flies were collected by sweep net from the vicinity of two small groups of "backyard" poultry (10-20 chickens per group) that had been identified as infected with exotic Newcastle disease virus (family Paramyxoviridae, genus avulavirus, ENDV) in Los Angeles County, CA, during the 2002-2003 END outbreak. Collected flies were subdivided into pools and homogenized in brain-heart infusion broth wit...